10x PBS

To make 10X PBS Buffer, dissolve the following reagents in 800 ml ultrapure water.

NaCl 80 g
KCl 2 g
Na2HPO4 14.4 g
KH2PO4 2.4 g


Adjust pH of PBS Buffer Solution to 7.4 with HCl.
Bring volume to 1 liter, autoclave or sterilize by filtration.

Molecular Cloning 
1X PBS (10 mM)
8 g NaCl 
0.2 g KCl 
1.44 g Na₂HPO₄ 
0.24 g KH₂PO₄ 

in 800 ml of distilled H₂O. 

Adjust the pH to 7.4 with HCl. Add H₂O to 1 liter. Sterilize by autoclave. 

10X PBS 

1. Dissolve the following in 800ml distilled H₂O.
   • 80g of NaCl
   • 2.0g of KCl
   • 14.4g of Na₂HPO₄
   • 2.4g of KH₂PO₄

1. Adjust pH to 7.4.
2. Adjust volume to 1L with additional distilled H₂O.
3. Sterilize by autoclaving.

Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.