蛋白質定量-二維電泳

2D Quant kit  (GE healthcare)

1. 0, 5. 10.15.20.25.30 uL BSA (2 mg/mL)for standard curve or1 uL 2D-sample
2. add 500 uL precipitant,vortex, put on the ice,for 2~3 min
3. add 500 uL co-precipitant, vortex, centrifugal 10,000 xg, for 5 min
4. Remove the supernatant, centrifugal 10,000 xg, for 1 min, as clean as possible.
5. add 400 uL dd Water+ 100 uL copper solution,
6. add 1mL color reagent mixture (A : B = 100 : 1) for 20 min
7. On sample, OD480, dd water as blank